Heterogeneity of hemolytic efficiency and isoelectric point of streptolysin O

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Heterogeneity of hemolytic efficiency and isoelectric point of streptolysin O.

Using thin-layer agarose gel isoelectric focusing overlaid with thin-layer erythrocyte agar plates, we found that crude streptolysin O (SLO) consisted of a variety of hemolytic components with different isoelectric points (pIs) and that the distribution of pIs in crude SLO was different even in samples which were produced from a single strain of Streptococcus pyogenes under similar conditions. ...

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Streptolysin-O was isolated from culture supernatants of group-A beta-hemolytic streptococci (Richards strain) by ammonium sulfate and polyethylene glycol precipitation, DEAE-ion exchange chromatography, preparative isoelectric focusing, and chromatography on Sephacryl S-300. Two forms of the toxin possessing similar hemolytic capacity were identified. The native toxin was a single polypeptide ...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1988

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.56.9.2474-2478.1988